Lysosomes play an important part in the function of a living cell. In this tutorial, you will learn about the structure and function of a lysosome, and how it uses enzymes to break down molecules, such as food molecules for the cell to use.
This lesson discusses the role of the lysosome in the cell.
Fluorescent pH probes are introduced into lysosomes which can then be detected by flow cytometry. The probe is typically "excited" at two or more wavelengths, causing it to fluoresce. The ratio of fluorescence can then be used to determine the pH in a lysosome (ratiometrics). This technique also works for other intracellular organelles.
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