cryo electron microscopy
Cryo-electron microscopy (cryo-EM) three-dimensional reconstruction technology can analyze the high-resolution three-dimensional structure of biological macromolecular complexes. Cryo-electron microscopy is a rapid freezing and fixation of aqueous biological samples, which are imaged by electron microscopy under liquid nitrogen / helium conditions. Cryofixation solves the problem of dehydration of biological samples and improves the radiation damage tolerance of biological samples. Low-dose irradiation technology can precisely control the electron radiation dose in the imaging area, thereby avoiding unnecessary radiation damage to the sample.
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