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fluorescence spectroscopy

fluorescence spectroscopy

Author: murphy wu

fluorescence spectroscopy

Fluorescence has two distinct stages, excitation stage and emission stage. It is a phenomenon where fluorescent chromophore absorbs a light photon, typically remains in an excited state for a few nanoseconds and then emits a lower energy photon. Both the efficiency of light absorption (ε) and the efficiency of photon emission from an excited fluorophore (Q) determine the intensity of a sample. Changes in either ε and/or Q will lead to changes in sample emission intensity.

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