Gel Electrophoresis Notes
Chapter 13 (pgs.345-346)
I. What is Gel Electrophoresis?
1. Each person’s DNA sequences (genome) is ___________________ from every other person’s DNA
2. This principle allows for each person to have their own _______________________________.
3. ____________________ allows scientists to analyze a person’s DNA quickly and cost-effectively.
4. Applications for Gel electrophoresis:
a. _____________________ with recipients in transplant programs
b. ____________________________ whose DNA may match evidence left at crime scenes
c. Exonerate wrongly accused people of crimes
d. _____________________ from crimes and catastrophes
e. _____________________ and other family relationships
II. How does DNA fingerprinting work?
1. Mapping out an entire person’s genome is too time consuming and too expensive
2. Look at specific ________________________________ and use them for identification
3. In every person’s genome there are ___________________________ of DNA
4. Use ___________________________ to cut at these landmarks repeating sections
III. How do we use DNA fragments to identify a person?
1. Each individual has ______________________ of fragments because of the way they were cut by
2. The goal of gel electrophoresis is to ______________those fragments by length so you can compare individuals.
3. _____________ are used to take samples of DNA and insert it into a ___ inside of an agorose gel.
4. The gel has a matrix that allows the fragments to move through the gel.
5. DNA is ___________________________, which means if expose it to a current the DNA will “run”
6. Not all fragments will move at the same speed
A. ________________DNA fragments will move ____________through the gel because they can
B. ________________DNA fragments will move _____________ through the gel because it is
7. The result is a barcode-like picture with the longer parts of DNA at the top and the short
8. Each person’s fragments are at different ______________ = each person has a different bar code or ______________________.
Why is each person’s DNA fingerprint unique to each individual?
What is another term for DNA fingerprinting?
Explain two ways Gel electrophoresis can be used:
Why is it important to have an electric current running through the agarose gel?