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hydrogen deuterium exchange

hydrogen deuterium exchange

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Author: murphy wu
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hydrogen deuterium exchange

Hydrogen–deuterium exchange, also called H–D or H/D exchange, is a chemical reaction, which can be used most easily to exchangeable protons and deuterons without any catalyst but in the presence of a suitable deuterium source. This often causes perdeuteration: hydrogen-deuterium exchange of all non-exchangeable hydrogen atoms in a molecule. It has built itself as a strong method for amide hydrogen/deuterium (H/D) exchanging of proteins monitored by mass spectrometry, which is for probing protein conformational dynamics and protein interactions.

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