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Leaf Disc Lab

Leaf Disc Lab

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Background to Leaf Disc Assay

 Go to  and read through the lab protocol provided here.  Sample results are also present.

The actual lab handout (that you will use) is at the bottom of this tutorial.  I will give you a hardcopy of it in class.

Source: EL Toolkit

Pre-lab Leaf Disc Assay for Photosynthesis

This is a short instructional video on the procedure for sinking leaf disks in a vacuum using a 10 ml syringe. The Floating Leaf disk assay is an excellent protocol for students investigating photosynthesis. The written protocol is available at

Source: ksbioteacher on YouTube

Leaf Disc Assay to Measure Photosynthesis

Handout for the Leaf Disc Assay lab.


Source: M. O'Mahony

Marksheet for lab

Marksheet that will be used to mark the Leaf Disc Assay lab.


Source: M. O'Mahony