nuclear magnetic resonance
In life, proteins are constantly moving, so static three-dimensional structures are often not enough to explain their biological functions. To truly elucidate the structural basis for protein functions, it is necessary to study the "dynamic structure" of protein molecules. One of the main features of liquid phase nuclear magnetic resonance (NMR) technology is the ability to study the three-dimensional structure of proteins in a state closer to the physiological environment (pH, salt concentration, temperature, etc.). This technique can be used to study the dynamics of protein multiple sites at the atomic level through the nucleus relaxation process.