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Recombinant DNA

Recombinant DNA

Author: Allyson Dunn
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Tutorial

Notes

 

QR-CodeRecombinant DNA Technology Notes

Chapter 13 (pgs.345-346)

 

Recombinant DNA:

Genetically engineered DNA made by ____________________ of DNA from different organisms.

Access lecture video at dunnclassroom.blogspot.com

 

 

 

 

A.      Forming recombinant DNA:

1)      Take “gene of interest” from one organism and__________ into the ____________of the organism you wish to alter.

2)      ______________ an unwanted gene from an organism.

 

Transgenic Organism/Genetically Modified Organism (GMO):

An organism whose __________ material has been ____________ or altered

 

 

 

 


B.      Forming a transgenic organism

1)      Insert the “gene of interest” by using a ___________.

2)      Two types of vectors: ________ vector and bacterial _____________.

 

 

 


C.      Using bacterial plasmids to form Recombinant DNA

1)      Remove plasmid from a bacterial cell

2)     

The plasmid is cut using _____________________.

 

·         ______  ______ form at both ends of the plasmid.

 

 

 

             

3)      The ______ ___ ________ (transgene) is removed from

the cell by restrictions enzymes.                                                                                                            

 

·         Sticky ends form at both ends

of the ____________.

 

 

 

 

 

4)     

The transgene is attached to the plasmid

 

 at the sticky ends by the use of the enzyme _________.

 

 

       
   
 
 

 

 

 

 

 


5)      The recombinant DNA plasmid is inserted into

a _____ ___________ cell which replicates and

expressed the new gene.

             

 

 

In your own words describe recombinant DNA

 

 

 

 

 

 

What does GMO stand for?

 

 

 

 

 

In your own words describe what a GMO is?

 

 

 

 

 

 

What enzyme connects the transgene with the bactierial plasmid?

 

 

 

 

 

 

What are sticky ends?

 

 

 

 

 

 

 

What enzyme cuts DNA at specific sequences?