ribo seq protocol
Ribosome profiling (Ribo-seq) is an established technique to detect translated transcript regions via next-generation sequencing (NGS). Ribo-seq closed the gap between RNA sequencing and proteomics by mapping the position of translating ribosomes over the entire transcriptome. Since its inception, scientists have employed Ribo-seq to answer questions from the detection of translated small open reading frames (OFRs) to the quantification of translational control.
