Theory behind Techniques

Theory behind Techniques


By working through the activities in this tutorial, you will be able to describe the technique, the major details of how it works and the results you get from:

  • restriction enzymes
  • PCR
  • gel electrophoresis
  • DNA sequencing.

There are more detailed expectations for the activities given in this lesson.

This tutorial involves working through interactive web activities to learn about four common techniques that you will be using in this Biotech unit.  Working through these simulations and taking basic notes (as instructed) as well as making reference to the lab manual you received in class. Some of the activities include quizzes - which will give you feedback on how well you understand the technology.

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Theory Lesson #1 - Part I

Begin with  http://www.dnai.org/b/index.html  to build context for Biotechnology.  Read thrrough and listen to the sections.

Go to: http://www.dnai.org/b/index.html  While you need to go through each of these "lessons", make detailed notes on:  Cutting and Pasting, Sorting and Sequencing, Ampllifying

Read through http://www.dnai.org/b/index.html

Source: HHMI, DNAi from Cold Spring Harbour

Restriction Enzymes- more details

Source: web sites cited above

Gel Electrophoresis - more details

Source: url's listed above

DNA Sequencing Activity

This activity is a simulation activity for DNA sequencing. It is an online activity. The attached pdf file gives the links and instructions.
Have fun!

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Source: Northwest Association for Biotechnical Research, 14 Aug 2012